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KMID : 0545120140240050661
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 5 p.661 ~ p.666
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Cell-Recycle Continuous Fermentation of Enterococcus faecalis RKY1 for Economical Production of Lactic Acid by Reduction of Yeast Extract Supplementation
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Lee Ryun-Kyung
Ryu Hwa-Won
Oh Hu-Rok
Kim Mi-Na
Wee Young-Jung
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Abstract
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Both lactic acid productivity and cell growth were linearly correlated with yeast extract supplementation in batch fermentation. During conventional continuous operation, although fresh feed was introduced into the bioreactor with a significantly low dilution rate (0.04 h-1), the amount of yeast extract employed was not enough to maintain the growth of microorganism. However, when the fresh feed contained 100 g/l glucose and 2 g/l yeast extract during cellrecycle continuous operation at a dilution rate of 0.04 h-1, more than 90 g/l lactic acid was continuously produced, with the average productivity of 3.72 g/l·h. In this experiment, 82 g of yeast extract (77% of reduction yield) could be reduced for the production of 1 kg of lactic acid compared with batch fermentation of a similar volumetric productivity.
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KEYWORD
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Cell-recycle, continuous fermentation, Enterococcus faecalis, lactic acid, yeast extract
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